Medical Mycology
Cellular and Molecular Techniques
Chapter One
Diagnosis of Candida infection in
tissue by immunohistochemistry
Malcolm D. Richardson, Riina Rautemaa and Jarkko Hietanen
1.1 Introduction
As a result of their large size, polysaccharide content and morphological diversity,
fungi can be detected readily and may also to a certain level be identified in
histological sections by conventional light microscopy. Application of immunohistochemical
techniques may, in several cases, be the only means of establishing an
accurate aetiological diagnosis in fixed-tissue sections because of morphological
similarities among the tissue forms of several fungal genera (e.g. aspergillosis,
fusariosis and scedosporiosis), when atypical forms of the fungus are present or
when fungal elements are sparse (Jensen et al., 1996; Jensen and Chandler, 2005).
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