Culture of Human Tumor Cells

This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.

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Title of ebook: Culture of Human Tumor Cells
ISBN: 9780471270621
parent-ISBN: 9780471438533
Publisher: Wiley-Liss
Internet download file size: 29825 kb
Pages: 435
Published: 02-2005
Released online for download: 02-25-2005
Editor: Pfragner, Roswitha
Editor: Freshney, R. Ian

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Chapter One

Growth of Human Lung Tumor Cells in Culture

Reen Wu

Center for Comparative Respiratory Biology and Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California, rwu@ucdavis.edu

1. Introductory Review 2 1.1. Cell Culture and Lung Cancer Development 2 1.2. Requirements for Growth Factors and Hormones for Lung Cancer Cells to Grow 3 2. Preparation of Media and Reagents 7 2.1. Preparation of HITES-Based Medium 7 2.2. Preparation of C and C-Modified Media 8 3. Safety Precautions 9 4. Step-by-Step Protocols 9 4.1. Tissue Handling and Shipping ... read full excerpt from Culture of Human Tumor Cells ebook

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