HPLC Methods for Recently Approved Pharmaceuticals
Chapter One
Abacavir
Molecular formula: [C.sub.14][H.sub.18][N.sub.6]O
Molecular weight: 286.33
CAS Registry No: 136470-78-5 (base), 188062-50-2 (sulfate)
Merck Index: 13,1
SAMPLE
Matrix: blood
Sample preparation: Condition a 1 mL 100 mg Bond Elut-C SPE cartridge with 1 mL
MeOH and 1 mL 100 mM pH 7.0 ammonium acetate buffer. Heat plasma at 58º for 1 h
to inactivate HIV. Vortex 800 µL plasma with 300 µL 2 µg/mL hexobarbital in 25 mM
pH 7.0 ammonium acetate buffer for 30 s and centrifuge at 18 000 g for 5 min. Add 1 mL
of the supernatant to the SPE cartridge, wash with 1 mL 100 mM pH 7.0 ammonium
acetate buffer, suck dry for 1 min, elute with 800 µL MeOH. Evaporate the eluate to
dryness under a stream of nitrogen at 40º and reconstitute the residue with 100 µL
mobile phase. Vortex for 30 s, centrifuge at 18 000 g for 3 min, and inject an 80 µL
aliquot.
HPLC VARIABLES
Guard column: 20 x 3.9 5 µm Polarity dC18 (Waters)
Column: 150 x 3.9 5 µm Polarity dC18 (Water ... read full excerpt from HPLC Methods for Recently Approved Pharmaceuticals ebook
