Chapter One
Basic Principles of Cell Culture
R. Ian Freshney
Centre for Oncology and Applied Pharmacology, Cancer Research UK Beatson
Laboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, UK,
i.freshney@ntlworld.com
1. Introduction 4
2. Types of Cell Culture 4
2.1. Primary Explantation Versus Disaggregation 4
2.2. Proliferation Versus Differentiation 4
2.3. Organotypic Culture 7
2.4. Substrates and Matrices 9
3. Isolation of Cells for Culture 9
3.1. Tissue Collection and Transportation 9
3.2. Biosafety and Ethics ...
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