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Home > Medical > Medical General & Other > Culture of Cells for Tissue Engineering-eBook
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Culture of Cells for Tissue Engineering
 
 
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This book offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures. Divided into two parts, the first section discusses in vitro cultivation procedures, and the second section covers a variety of tissue engineering applications. Written by leading experts in the field, each chapter gives a brief overview of basic principles, followed by detailed cell culture and tissue engineering procedures that incorporate state-of-the-art examples and case studies. Each chapter includes stepwise protocols for representative well-established methods.
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Title of ebook: Culture of Cells for Tissue Engineering
ISBN: 9780471741800
Publisher: Wiley-Liss
Internet download file size: 5652 kb
Pages: 512
Released online for download: 02-02-2006
Editor: Vunjak-Novakovic, Gordana
Editor: Freshney, R. Ian


Chapter One
Basic Principles of Cell Culture

R. Ian Freshney
Centre for Oncology and Applied Pharmacology, Cancer Research UK Beatson Laboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, UK, i.freshney@ntlworld.com
1. Introduction 4
2. Types of Cell Culture 4
2.1. Primary Explantation Versus Disaggregation 4 2.2. Proliferation Versus Differentiation 4 2.3. Organotypic Culture 7 2.4. Substrates and Matrices 9
3. Isolation of Cells for Culture 9
3.1. Tissue Collection and Transportation 9 3.2. Biosafety and Ethics ... read full excerpt from Culture of Cells for Tissue Engineering ebook




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