Chapter One
Basic Principles of Cell Culture
R. Ian Freshney
Centre for Oncology and Applied Pharmacology, Cancer Research UK Beatson
Laboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, UK,
i.freshney@ntlworld
1. Introduction 4
2. Types of Cell Culture 4
2.1. Primary Explantation Versus Disaggregation 4
2.2. Proliferation Versus Differentiation 4
2.3. Organotypic Culture 7
2.4. Substrates and Matrices 9
3. Isolation of Cells for Culture 9
3.1. Tissue Collection and Transportation 9
3.2. Biosafety and Ethics 10
3.3. Record Keeping 11
3.4. Disaggregation and Primary Culture 11
4. Su ... read full excerpt from Culture of Cells for Tissue Engineering ebook
