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 New Frontiers in Ultrasensitive Bioanalysis: Advanced Analytical Chemistry Applications in Nanobiotechnology, Single Molecule Detection, and Single Ce eBook

New Frontiers in Ultrasensitive Bioanalysis: Advanced Analytical Chemistry Applications in Nanobiotechnology, Single Molecule Detection, and Single Ce


eBook Publisher: John Wiley & Sons
Imprint: Wiley-Interscience

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An overview of current research and developments in ultrasensitive bioanalysis

New platforms of ultrasensitive analysis of biomolecules and single living cells using multiplexing, single nanoparticle sensing, nano-fluidics, and single-molecule detection are advancing every scientific discipline at an unprecedented pace. With chapters written by a diverse group of scientists working in the forefront of ultrasensitive bioanalysis, this book provides an overview of the current status and an in-depth understanding of the objectives and future research directions of ultrasensitive bioanalysis. Spanning a wide spectrum of new research approaches, this book: Introduces new theories, ideas, methodologies, technologies, and applications of ultrasensitive bioanalysis in a wide variety of research fields Includes background, fundamentals, and descriptions of instrumentation and techniques behind every experimental design and approach to help readers explore the promising applications of new tools Covers single molecule detection (SMD), single living cell analysis, multi-functional nanoparticle probes, miniaturization, multiplexing, quantitative and qualitative analysis of metal ions and small molecules, and more Discusses techniques such as single molecule microscope and spectroscopy, single nanoparticle optics, single nanoparticle sensors, micro- and nano-fluidics, microarray detection, ultramicroelectrodes, electrochemiluminescence, mass spectrometry, and more

This book will be a useful resource and an inspiration for scientists and graduate and undergraduate students in a wide variety of research fields, including chemistry, biology, biomedical science and engineering, and materials science and engineering.

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Title of eBook: New Frontiers in Ultrasensitive Bioanalysis: Advanced Analytical Chemistry Applications in Nanobiotechnology, Single Molecule Detection, and Single Ce
Release Date: 06-04-2007
Publisher: Wiley-Interscience

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New Frontiers in Ultrasensitive Bioanalysis: Advanced Analytical Chemistry Applications in Nanobiotechnology, Single Molecule Detection, and Single Ce


Chapter One

IS ONE ENOUGH?

ANDREW C. BEVERIDGE, JAMES H. JETT, AND RICHARD A. KELLER

1.1. INTRODUCTION

A concern often expressed about single-molecule measurements can be stated as, "Single-molecule measurements are 'cool' but what good are they if you have to measure a million single molecules to estimate the properties of the system?" This is a reasonable concern. Thus, a major focus of this chapter is modeling the effect of sample size (typically a few molecules) on the accuracy of analytical measurements and the extrapolation of properties measured at the single-molecule level to properties derived from ensemble measurements. We include a summary of DNA fingerprinting of single viral particles and bacterial cells that demonstrates that only a few measurements are required. The contrast between single-molecule and bulk measurements is that single-molecule measurements yield physical properties of individual molecules whereas individual molecular properties are difficult or impossible to obtain from bulk measurements; often the heterogeneous nature of single measurements is masked in bulk systems. We finish with (a) a description of "virtual sorting," where the data stream is sorted instead of the sample stream, and (b) the implementation of virtual sorting to statistical chemistry to either eliminate or reduce the necessity to purify a sample.

1.1.1. Significance of a Single-Molecule Measurement

There is a fundamental postulate of statistical mechanics that states, "The (long) time average of a mechanical property

...

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